Study of product effects on the production of collagen neo-synthesis in human fibroblasts.
IEC Espagne Institute of Spanish Clinical Expertise
Study of product effects on the production of collagen neo-synthesis in human fibroblasts.
Report: In Vitro Clinical Study
At the request of the INSTITUT D’EXPERTISE CLINIQUE – ESPAGNE, we have evaluated the effect of LIVING SILICA in the following parameters:
Synthesis of Collagen in Human Fibroblasts
Cytotoxicity in Human Fibroblasts
Evaluation of Cytotoxicity
The results are presented in the following table:
| Optical density | % | |
| (570nm) | ||
| Negative Test | 0.512 ± 0.03 | - |
| Living Silica ® (0.2%) | 0.508 ± 0.04 | ns |
| Living Silica ® (0.5%) | 0.500 ± 0.06 | ns |
| Living Silica ® (1%) | 0.514 ± 0.04 | ns |
| NS: Not Significant |
The results show that LIVING SILICA does not present cytotoxicity to human fibroblasts in culture when used at 0.2%, 0.5%, and 1. %.
The separation and identification of hydroxyproline were performed by reversed-phase HPLC. Fluorescence peaks, after integration, used to calculate the concentration of hydroxyproline in the medical culture.
| Concentration of hydroxyproline (mg/ml) | % increase | |
| Negative Test | 1.64 ± 0.10 | - |
| Living Silica ® 0.2% | 1.79 ± 0.13 | 9 |
| Living Silica ® 0.5% | 1.86 ± 0.08 | 13 |
| Living Silica ® 1% | 1.95 ± 0.10 | 19 |
The results show that LIVING SILICA significantly increments the level of collagen produced by the human fibroblast cultures by comparison with the control.
In the experimental conditions adopted for the product LIVING SILICA :
Induces a significant increase in collagen levels of 13% and 19% respectively for the concentrations of 0.5% and 1% in cultured human fibroblasts compared with the control.
Does not induce cytotoxicity in the concentrations (0.2%, 0.5%, and 1%)
In conclusion, the LIVING SILICA product induces a significant increase in the index in cultured human fibroblasts.
Product being investigated: Organic Silica (ref: LIVING SILICA)
Spanish I.E.C. Code: E080043 130003
Experiment Protocol: No. E080043PE, of February 26, 2008
Authentication
Study No. E080043RD
Study Sponsor
Mr. J.C. Hierro Alonso
Director-General Master’s in microbiology
Silicium Laboratories SL
I.E.C. Spain
Cerviago 5 C/Caspe, 104, Bajo 33875 Tineo 08010 Barcelona, Spain.
IEC Espagne Institute of Spanish Clinical Expertise
Study of product effects on the production of collagen neo-synthesis in human fibroblasts.
Report: In Vitro Clinical Study
At the request of the INSTITUT D’EXPERTISE CLINIQUE – ESPAGNE, we have evaluated the effect of LIVING SILICA in the following parameters:
Synthesis of Collagen in Human Fibroblasts
Cytotoxicity in Human Fibroblasts
The results are presented in the following table:
| Optical density | % | |
| (570nm) | ||
| Negative Test | 0.512 ± 0.03 | - |
| Living Silica ® (0.2%) | 0.508 ± 0.04 | ns |
| Living Silica ® (0.5%) | 0.500 ± 0.06 | ns |
| Living Silica ® (1%) | 0.514 ± 0.04 | ns |
| NS: Not Significant |
The results show that LIVING SILICA does not present cytotoxicity with regard to any human fibroblasts in culture when used to 0.2%, 0.5%, and 1.%.
The separation and identification of hydroxyproline were performed by reversed-phase HPLC. Fluorescence peaks, after integration, used to calculate the concentration of hydroxyproline in the medical culture.
| Concentration of hydroxyproline (mg/ml) | % increase | |
| Negative Test | 1.64 ± 0.10 | - |
| Living Silica ® 0.2% | 1.79 ± 0.13 | 9 |
| Living Silica ® 0.5% | 1.86 ± 0.08 | 13 |
| Living Silica ® 1% | 1.95 ± 0.10 | 19 |
The results show that LIVING SILICA significantly increments the level of collagen produced by the human fibroblast cultures by comparison with the control.
Conclusion
In the experimental conditions adopted for the product LIVING SILICA :
Induces a significant increase in collagen levels of 13% and 19% respectively for the concentrations of 0.5% and 1% in cultured human fibroblasts compared with the control.
Does not induce cytotoxicity in the concentrations (0.2%, 0.5%, and 1%)
In conclusion, the LIVING SILICA product induces a significant increase in the index in cultured human fibroblasts.
Product being investigated: Organic Silica (ref: LIVING SILICA)
Spanish I.E.C. Code: E080043 130003
Experiment Protocol: No. E080043PE, of February 26, 2008
Authentication
Study No. E080043RD
Study Sponsor
Mr. J.C. Hierro Alonso
Director-General Master’s in microbiology
Silicium Laboratories SL
I.E.C. Spain
Cerviago 5 C/Caspe, 104, Bajo 33875 Tineo 08010 Barcelona, Spain.
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