IEC Espagne Institute of Spanish Clinical Expertise
Study of product effects on the production of collagen neo-synthesis in human fibroblasts.
Report: In Vitro Clinical Study
At the request of the INSTITUT D’EXPERTISE CLINIQUE – ESPAGNE, we have evaluated the effect of Living Silica ® in the following parameters:
- Synthesis of Collagen in Human Fibroblasts
- Cytotoxicity in Human Fibroblasts
Evaluation of Cytotoxicity
The results are presented in the following table:
| Optical density | % | |
| (570nm) | ||
| Negative Test | 0.512 ± 0.03 | - |
| Living Silica ® (0.2%) | 0.508 ± 0.04 | ns |
| Living Silica ® (0.5%) | 0.500 ± 0.06 | ns |
| Living Silica ® (1%) | 0.514 ± 0.04 | ns |
| NS: Not Significant |
The results show that Living Silica ® does not present cytotoxicity with regard to any human fibroblasts in culture when used to 0.2%, 0.5%, and 1.%.
Evaluation of the Collagen Index
The separation and identification of hydroxyproline were performed by reversed-phase HPLC. Fluorescence peaks, after integration, used to calculate the concentration of hydroxyproline in the medical culture.
| Concentration of hydroxyproline (mg/ml) | % increase | |
| Negative Test | 1.64 ± 0.10 | - |
| Living Silica ® 0.2% | 1.79 ± 0.13 | 9 |
| Living Silica ® 0.5% | 1.86 ± 0.08 | 13 |
| Living Silica ® 1% | 1.95 ± 0.10 | 19 |
The results show that Living Silica ® significantly increments the level of collagen produced by the human fibroblast cultures by comparison with the control.
Conclusion
In the experimental conditions adopted for the product Living Silica ® :
- Induces a significant increase in collagen levels of 13% and 19% respectively for the concentrations of 0.5% and 1% in cultured human fibroblasts compared with the control.
- Does not induce cytotoxicity in the concentrations (0.2%, 0.5%, and 1%)
In conclusion, the Living Silica ® product induces a significant increase in the index in cultured human fibroblasts.
Product being investigated: Organic Silica (ref: Living Silica ®)
Spanish I.E.C. Code: E080043 130003
Experiment Protocol: No. E080043PE, of February 26, 2008
Authentication
Study No. E080043RD
Study Sponsor
Mr. J.C. Hierro Alonso
Director-General Master’s in microbiology
Silicium Laboratories SL
I.E.C. Spain
Cerviago 5 C/Caspe, 104, Bajo 33875 Tineo 08010 Barcelona, Spain.
IEC Espagne Institute of Spanish Clinical Expertise
Study of product effects on the production of collagen neo-synthesis in human fibroblasts.
Report: In Vitro Clinical Study
At the request of the INSTITUT D’EXPERTISE CLINIQUE – ESPAGNE, we have evaluated the effect of Living Silica ® in the following parameters:
- Synthesis of Collagen in Human Fibroblasts
- Cytotoxicity in Human Fibroblasts
Evaluation of Cytotoxicity
The results are presented in the following table:
| Optical density | % | |
| (570nm) | ||
| Negative Test | 0.512 ± 0.03 | - |
| Living Silica ® (0.2%) | 0.508 ± 0.04 | ns |
| Living Silica ® (0.5%) | 0.500 ± 0.06 | ns |
| Living Silica ® (1%) | 0.514 ± 0.04 | ns |
| NS: Not Significant |
The results show that Living Silica ® does not present cytotoxicity with regard to any human fibroblasts in culture when used to 0.2%, 0.5%, and 1.%.
Evaluation of the Collagen Index
The separation and identification of hydroxyproline were performed by reversed-phase HPLC. Fluorescence peaks, after integration, used to calculate the concentration of hydroxyproline in the medical culture.
| Concentration of hydroxyproline (mg/ml) | % increase | |
| Negative Test | 1.64 ± 0.10 | - |
| Living Silica ® 0.2% | 1.79 ± 0.13 | 9 |
| Living Silica ® 0.5% | 1.86 ± 0.08 | 13 |
| Living Silica ® 1% | 1.95 ± 0.10 | 19 |
The results show that Living Silica ® significantly increments the level of collagen produced by the human fibroblast cultures by comparison with the control.
Conclusion
In the experimental conditions adopted for the product Living Silica ® :
- Induces a significant increase in collagen levels of 13% and 19% respectively for the concentrations of 0.5% and 1% in cultured human fibroblasts compared with the control.
- Does not induce cytotoxicity in the concentrations (0.2%, 0.5%, and 1%)
In conclusion, the Living Silica ® product induces a significant increase in the index in cultured human fibroblasts.
Product being investigated: Organic Silica (ref: Living Silica ®)
Spanish I.E.C. Code: E080043 130003
Experiment Protocol: No. E080043PE, of February 26, 2008
Authentication
Study No. E080043RD
Study Sponsor
Mr. J.C. Hierro Alonso
Director-General Master’s in microbiology
Silicium Laboratories SL
I.E.C. Spain
Cerviago 5 C/Caspe, 104, Bajo 33875 Tineo 08010 Barcelona, Spain.
